背景概念
生物学真实损伤和实验引入损伤间的差异。
DNA易受多种损伤，其中之一就是氧化损伤。随着时间的推移，这种类型的损害会逐渐累积，破坏修复系统，导致健康问题，最终导致疾病。
在生理环境下，一旦某一个碱基发生改变如果没有被错配修复蛋白处理掉，这种错配可以传递给子代，形成突变，一个真实的突变正模板链和对应负模板链应同时被替换。
如果碱基改变是发生在实验阶段，那么其对应链不会发生改变，正链发生G>8-oxoG的改变时，由于其可与A配对，易被测序仪读成T，但对应的负链C碱基，仍会被读为C，而不是A
| 正负链 | 真实突变 | 假突变 |
|---|---|---|
| 正链：5’-3’ | 5’- ATC$\color{red}{G}$ATCG-3 | 5’- ATC$\color{red}{G}$ATCG-3 |
| 负链：3’-5’ | 3’- TAG$\color{red}{A}$TAGC-5 | 3’- TAG$\color{red}{C}$TAGC-5 |
NGS识别氧化损伤的技术基础
处理方式
参考文献[16]
参考资料
文献
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